Search results for "Wollaston prism"
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Recent Advances in 3D Structured Illumination Microscopy
2018
In structured illumination microscopy (SIM) the sample under investigation is illuminated using a structured illumination (SI) pattern. This SI pattern encodes high spatial frequencies of fine features within the sample, which usually are not transferred by the conventional three-dimensional (3D) optical transfer function (OTF) of the imaging system and fills the missing cone of frequencies in the OTF for better discrimination of the out-of-focus light. Thereby, SIM provides super-resolution (SR) performance beyond the diffraction limit and optical-sectioning (OS) capability with the use of data post-processing approaches. 3D structured patterns that include lateral and axial variations in …
Tunable structured illumination system based on a Wollaston prism
2018
Experimental verification of a simple illumination system to generate a 1D structured pattern with tunable modulation frequency is shown based on a Wollaston prism illuminated by the diffracted field of an incoherent linear source.